Fig. 5

CircCRIM1 positively regulates the expression of CRIM1 through miR-145-5p. QRT-PCR showed that there was a positive correlation relationship between circCRIM1 and CRIM1 (a). CRIM1 expression was obviously positively influenced by circCRIM1 (b). TargetScanHuman predicted miR-145-5p might combine with 3’UTRs of CRIM1 and Circular RNA Interactome predicted miR-145-5p might bind with circCRIM1(c). Dual luciferase reporter assay verified it (d). MiR-145-5p treatment reversed the expression of CRIM1 after the over-expression of circCRM1(f). MiR-145-5p mimics efficiency was verified (e). Three separate experiments were conducted; data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001