Fig. 2

ICA administration improved impaired seminiferous tubule in diabetic rats. A Hematoxylin and eosin staining of testicular tissue. The lower panes show partial amplification of upper panes. In each seminiferous tubule, the black arrow points to a representative spermatogonia, the red arrow, primary spermatocyte, and the green arrowhead, Sertoli cell. In addition, the green curves present regions used to analyze the cross-sectional area of seminiferous tubule whose value displayed beside. B The area of seminiferous tubules from different treatment groups. C Quantification of spermatogonia, primary spermatocytes and Sertoli cells from different treatment groups. N = 6. Bars assigned with different superscripts mean significant difference (p < 0.05)