Table 1 Overview of the outcome parameters assessed at specific time points during the preconception care intervention period (PCCI)
PCCI week 0 | PCCI week 2 | PCCI week 4 | PCCI week 6 | ||
|---|---|---|---|---|---|
Metabolic health | Insulin sensitivity testa (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ |
Glucose tolerance testa (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
Serumb - Glucose - Insulin - NEFA - Cholesterol - Triglycerides - ALT (n = 7–8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
Fertility | Oocyte recovery rateb (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ |
Lipid droplet volume in oocytesb (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
ROS in oocytesb (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
Mitochondrial activity in oocytesb (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
Localization of active mitochondria in mature oocytesb (n = 8 mice/treatment group/time point) | ✓ | ✓ | ✓ | ✓ | |
mtDNA copy numbers in oocytesb (n = 3–5 mice/treatment group/time point) | ✓ | ✓ | ✓ | ||
Mitochondrial ultrastructural abnormalities (TEM)b in cumulus cells and oocytes (n = 3–5 mice/treatment group/time point) | ✓ | ✓ | |||
Pregnancy rates (n = 8 mice/treatment group) | ✓ |