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Table 1 Overview of the outcome parameters assessed at specific time points during the preconception care intervention period (PCCI)

From: Diet normalization or caloric restriction as a preconception care strategy to improve metabolic health and oocyte quality in obese outbred mice

   PCCI week 0 PCCI week 2 PCCI week 4 PCCI week 6
Metabolic health Insulin sensitivity testa
(n = 8 mice/treatment group/time point)
Glucose tolerance testa
(n = 8 mice/treatment group/time point)
Serumb
- Glucose
- Insulin
- NEFA
- Cholesterol
- Triglycerides
- ALT
(n = 7–8 mice/treatment group/time point)
Fertility Oocyte recovery rateb
(n = 8 mice/treatment group/time point)
Lipid droplet volume in oocytesb
(n = 8 mice/treatment group/time point)
ROS in oocytesb
(n = 8 mice/treatment group/time point)
Mitochondrial activity in oocytesb
(n = 8 mice/treatment group/time point)
Localization of active mitochondria in mature oocytesb
(n = 8 mice/treatment group/time point)
mtDNA copy numbers in oocytesb
(n = 3–5 mice/treatment group/time point)
 
Mitochondrial ultrastructural abnormalities (TEM)b in cumulus cells and oocytes
(n = 3–5 mice/treatment group/time point)
  
Pregnancy rates
(n = 8 mice/treatment group)
   
  1. NEFA non-esterified fatty acids, ALT alanine aminotransferase, ROS reactive oxygen species, TEM transmission electron microscopy
  2. aSame mice were used to determine the selected outcome parameters over all PCCI time points
  3. b Same mice were used per PCCI to analyze the selected outcome parameters