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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: DNA methylation and gene expression changes in mouse pre- and post-implantation embryos generated by intracytoplasmic sperm injection with artificial oocyte activation

Fig. 4

The imprinted gene expression in embryos. A Transcript abundance of Igf2r imprinted genes in blastocysts obtained after ICSI, ICSI-AOA or dICSI-AOA. Each spot represents one experiment. B Relative expression level of Igf2r imprinted genes was validated by quantitative PCR. Each spot represents one experiment. * P < 0.05 was indicated significantly different. C Heat map of expression of key genes related to epigenetic reprogramming. D QPCR analysis of gene expression at Bt, E6.5, E7.5, and E8. Left panel showed Igf2r expression during embryo development stages. Right panel showed Airn expression during embryo development stages. The value at E8 was set to 100. The mean of three biological replicates is given with SD. Bt, blastocyst

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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