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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: DNA methylation and gene expression changes in mouse pre- and post-implantation embryos generated by intracytoplasmic sperm injection with artificial oocyte activation

Fig. 1

Calcium oscillation pattern analysis. A Representative calcium oscillation patterns for the calcium frequency scoring following normal mouse spermatozoa. The frequency score per oocyte is given based on the number of calcium rises seen during the 1 h measuring period: ‘+++’, > 8 spikes; ‘++’, 4–8 spikes; ‘+’, 1-3 spikes; ‘0’, no calcium spikes seen. B Frequency scoring of the calcium oscillations following normal mouse spermatozoa or oocyte activation-deficient mouse spermatozoa ICSI with different concentration of ionomycin. * indicates distribution of frequency pattern significant differences compared to ICSI group, P < 0.05

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