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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: WEE1 promotes endometriosis via the Wnt/β-catenin signaling pathway

Fig. 2

WEE1 promotes ESC migration and limits apoptosis in ESCs. A Western blot analysis of WEE1 protein levels in WEE1 overexpressing or knockdown ESCs. WEE1 protein levels were normalized against β-actin levels, and quantitative analysis of protein content was performed using ImageJ. Relative WEE1 protein levels are presented as mean ± SD. B The wound healing assay was used to examine the effects of WEE1 overexpression and knockdown on ESC migration. Scale bar: 200 μm. C The Transwell assay was used to determine the effect of WEE1 overexpression and knockdown on ESC migration. Scale bar: 50 μm. D The TUNEL assay was performed to examine the role of WEE1 overexpression and knockdown on the apoptosis of ESCs. Scale bar: 200 μm. ** p < 0.01; *** p < 0.001 compared to control cells

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