Alterations of miR-16, miR-let-7a and their target genes expression in human blastocysts following vitrification and re-vitrification

Table 2 Primers used for quantitative gene expression

Gene name	Primer sequence
ITGβ3	F: AGTAACCTGCGGATTGGCTTC
ITGβ3	R: GTCACCTGGTCAGTTAGCGT
BCL-2 (Homo sapiens BCL2 apoptosis regulator (BCL2), transcript variant alpha)	F: GAGAAATCAAACAGAGGCCG
	R: CTGAGTACCTGAACCGGCA
BAX	F: CCCGAGAGGTCTTTTTCCGAG
BAX	R: CCAGCCCATGATGGTTCTGAT
B2M	F: GACTGGTCTTTCTATCTCTTGTAC
B2M	R: ATGTCTCGATCCCACTTAACTATC
miR-16	F: GTATACTAGCAGCACGTAAAT
miR-16	R: GTGCAGGGTCCGAGGT
miR-let-7a	F: GTATACTGAGGTAGTAGGTTG
miR-let-7a	R: GTGCAGGGTCCGAGGT
SNORD	F:AGATTTAACAAAAATTCGTCAC
SNORD	R:GAGCAGGGTCCGAGGT

ISSN: 1477-7827