Fig. 3

The effects of circ_0043532 knockdown on cell proliferation and promoted cell cycle arrest and apoptosis in PCOS GCs and KGN cells were reversed by miR-182 inhibition. (A) The expression of miR-182 in PCOS GCs and non-PCOS GCs was examined by qRT-PCR. (B) The expression of miR-182 in IOSE80 cells and KGN cells was measured with qRT-PCR. (C) The level of miR-182 in PCOS GCs and KGN cells transfected with in-miR-con or in-miR-182 was detected by qRT-PCR. (D-M) si-con, si-circ_0043532, si-circ_0043532 + in-miR-con or si-circ_0043532 + in-miR-182 was transfected into PCOS GCs and KGN cells. (D and E) The expression level of miR-182 in transfected PCOS GCs and KGN cells was examined through qRT-PCR analysis. (F and G) The proliferation of PCOS GCs and KGN cells was evaluated by EdU assay and MTT assay. (H-K) Cell cycle and cell apoptosis in PCOS GCs and KGN cells were analyzed by flow cytometry analysis. (L and M) The protein level of BAX in PCOS GCs and KGN cells was measured via western blot assay. *P < 0.05