Follistatin supplementation induces changes in CDX2 CpG methylation and improves in vitro development of bovine SCNT preimplantation embryos

Table 1 Transcription factor motif analysis of methylated CpG sites effected by follistatin treatment

Fragment	Location (bp from TSS)	CpG methylation in response to FST	Putative transcription factors^a
P1	-1374	↓	Oct1 RXRF HICF HAND HESF MYOD DEAF
P2	-279	↓	OCT1 STEM
	-243	↑	ZF5F E2FF
	-163	↓	AP2F ZFXY
	-23	↓	P53F
	20	↑	HNFP NRSF
	122	↓	WHNF ZTRE
I	3558	↓	KLFS ZF02 SAL BED EGR GLIF NDPK

Abbreviations: bp base pair, TSS transcription start site, FST follistatin
^aPutative transcription factor binding sites that overlap differentially methylated CpG sites

ISSN: 1477-7827