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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Follistatin supplementation induces changes in CDX2 CpG methylation and improves in vitro development of bovine SCNT preimplantation embryos

Fig. 2

Follistatin supplementation improves blastocyst development and upregulates CDX2 expression in bovine SCNT embryos. Activated SCNT zygotes were cultured with or without follistatin (10 ng/ml) for 72h. Then, embryos were cultured in follistatin free media until d 7. A Percentage of embryos that developed to the blastocyst stage. B QRT-PCR analysis of CDX2 mRNA abundance in blastocysts in response to follistatin treatment. CDX2 mRNA expression was normalized relative to the abundance of RPS18 as a housekeeping gene. Data are expressed as mean ± SEM. Values with different superscripts indicate significant differences (P < 0.05)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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