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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Follistatin supplementation induces changes in CDX2 CpG methylation and improves in vitro development of bovine SCNT preimplantation embryos

Fig. 1

Study design and bovine CDX2 gene structure. A To investigate the effects of follistatin on early development of somatic cell nuclear transfer (SCNT) embryos and CDX2 methylation status in blastocyst, activated SCNT zygotes were cultured with or without follistatin (10 ng/ml) during IVC1, then 8-16 cell embryos were cultured in follistatin free media until day 7, when percentage of embryos developing to blastocyst stage were recorded. Then blastocysts were used for extraction of total RNA for CDX2 gene expression analysis and genomic DNA for bisulfite sequencing analysis. B Schematic illustration of bovine CDX2 gene showing the regulatory elements of the gene (proximal promoter and intron 1) and position of the fragments selected for analysis (P1, P2 and I)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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