Fig. 4

Meiotic divisions appeared to be normal in spermatocytes from Spindoc-null mice. (A) Immunofluorescence staining of SYCP3 (red) and γH2AX (green) on chromosome spreads of spermatocytes from the testes of WT and KO males at P21. DAPI indicates the nucleus. Scale bar, 10 μm. (B) Quantitative counting of the proportion of meiotic stages in WT and KO spermatocytes. Distinct meiotic stages (leptotene, zygotene, pachytene, diplotene and diakinesis) were identified by the characteristic patterns as shown in (A). For each genotype, three mice were analyzed. p-values were calculated by Student’s t-test. Data are presented as the mean ± SD; n.s., not statistically significant. (C) Immunofluorescence staining of SYCP3 (red) and SYCP1 (green) on chromosome spreads of spermatocytes from the testes of WT and KO males at P21. DAPI indicates the nucleus. Scale bar, 10 μm. (D) HE staining of the testes from the WT and KO mice at the age of 21 days. Areas with dash boxes were displayed with higher magnification on the right panel. Scale bar, 20 μm