TRIB3 regulates FSHR expression in human granulosa cells under high levels of free fatty acids

Table 1 Oligonucleotide primers used for qPCR analysis

Target gene	Primer sequence (5’ → 3’)	Sense Anti-sense*	Product size (bp)	Efficiency (%)
FSHR^a	GGC CAT GCT CAT CTT CAC TG	S	157
FSHR^a	ATA GAG GAA GGG GTT GGC AC	AS	157	98.84
*TRIB3*	GTCCGAGTGAAAAAGGCGTA	AS	158
*TRIB3*	TGCCCTACAGGCACTGAGTA	S	158	98.82
*ATF4*^b	ATGACCGAAATGAGCTTCCTG	S	153
*ATF4*^b	GCTGGAGAACCCATGAGG	AS	153	99.33
*CYP17A1*^a	GCT GCT TAC CCT AGC TTA TTT GT	S	174
*CYP17A1*^a	ACC GAA TAG ATG GGG CCA TAT TT	AS	174	98.03
*CYP19A1*^a	CGA AAG TGC TAT CGT GGT T	S	178
*CYP19A1*^a	TGT GGA AAT CCT GCG TCT	AS	178	98.84
*GAPDH*^a	CAC ATC GCT CAG ACA CCA TG	S	198
*GAPDH*^a	TGA CGG TGC CAT GGA ATT TG	AS	198	98.84

Note: * S, sense; AS, anti-sense.
^aXU P, HUANG B-Y, ZHAN J-H et al. Insulin Reduces Reaction of Follicular Granulosa Cells to FSH Stimulation in Women With Obesity-Related Infertility During IVF. The Journal of Clinical Endocrinology & Metabolism 2018, 104: 2547-2560
^bDI F, LIU J, LI S et al. Activating transcriptional factor 4 correlated with obesity and insulin resistance in polycystic ovary syndrome. Gynecol Endocrinol 2019, 35: 351-355

ISSN: 1477-7827