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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: TRIB3 regulates FSHR expression in human granulosa cells under high levels of free fatty acids

Fig. 4

Western blot analysis of the Akt/GSK3β pathway following TRIB3 knockdown in KGN cells. A Western blot analysis was performed in shRNA-control and shRNA-TRIB3 cells treated with PA; tubulin was used as the loading control. B PA increased TRIB3 levels in a concentration-dependent manner, TRIB3 knockdown reduced this effect. C TRIB3 knockdown prevented PA-induced FSHR decrease. D TRIB3 knockdown blocked PA-induced decrease of p-AKT. E TRIB3 knockdown blocked PA-induced increase in p-GSK3β. (b–e) P529, together with rFSH and PA, enhanced the reduction of FSHR, p-Akt and the increase of p-GSK3β protein expression. F PA treatment inhibited E2 production, TRIB3 knockdown reversed this effect; P529 enhanced it

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