Fig. 3

Global RNA analysis of GV oocytes developing in vivo and in vitro. a PCA of GV oocytes developing in vivo (IVV), in vitro (IVG), and in vitro with NT-4 (IVG-NT). b Gene-expression profiles of genes necessary for oogenesis (Lhx8, Nobox, Sohlh2, Zp1, Zp3, Foxo3) and fully grown oocyte (Suv39h1, Zp4). The transcription level was calculated by the fragments per kilobase of exon model per million mapped fragments (FPKM). Bars at each point indicate the SD based on three independent experiments. *, P < 0·05 IVV vs. IVG, IVV vs. IVG-NT. c Heatmap analysis of gene expression profiles of oocytes in IVV, IVG, IVG-NT groups. d GSEA enrichment plots of KEGG signaling pathways of upregulated and downregulated different-expressed genes (DEGs) in IVT oocytes compared with IVV oocytes. Blue represents upregulated pathways; orange represents downregulated pathways. e KEGG enrichment analysis of upregulated DEGs in IVT oocytes compared with IVV oocytes using DAVID. f KEGG enrichment analysis of downregulated DEGs in IVT oocytes compared with IVV oocytes using DAVID. g Transcriptional level of TRKB receptor (Ntrk2) gene at different folliculogenesis stages with qRT-PCR experiments. *, P < 0·05 IVV vs. IVG of Day 10 oocytes. h, i, j Quantitative RT-PCR detection of Cacna1s (h), Orail (i), Prkaca (j) transcripts in oocytes from Day 10 follicles. *, P < 0·05. Each group contains three samples from three independent experiments