Fig. 6

MiR-3935 expression correlates with RGS2 mRNA level. A Immunohistochemistry (IHC) staining for RGS2 by using paraffin-embedded sections of placentas samples from patients with PE and from normal pregnancies. IgG was used as a negative control (up). N = 15; bar, 100 μm. B IHC score for RGS2 in (A). **p < 0.01 versus normal group (Normal). C The mRNA expression of RGS2 was measured using qRT-PCR in placentas samples from 15 patients with PE and 15 women with normal pregnancies. **p < 0.01 versus normal group (Nor.). Data represent the mean ± SD of three independent experiments. D The correlation between miR-3935 and RGS2 in placentas was determined by Pearson’s correlation coefficient (r: 0.9746, p < 0.01). N = 15. E A working model for miR-3935/TRAF6/RGS2 signal axis in human trophoblast EMT