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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: MicroRNA-3935 promotes human trophoblast cell epithelial-mesenchymal transition through tumor necrosis factor receptor-associated factor 6/regulator of G protein signaling 2 axis

Fig. 4

RGS2 exerts the effect of miR-3935 on trophoblast cells EMT. A Western blot analysis for E-Cadherin, Vimentin and N-Cadherin expression levels in miR-3935 mimics-transfected or negative control-transfected (NC) HTR-8/SVneo cells. B Immunofluorescent staining of E-Cadherin-derived signal in HTR-8/SVneo cells transfected with miR-3935 mimics or negative control (NC). Nuclei were stained with DAPI. C Western blot analysis for E-Cadherin, Vimentin and N-Cadherin expression levels in miR-3935 inhibitor-transfected or negative control-transfected (NC) HTR-8/SVneo cells. D Western blot analysis for E-Cadherin, Vimentin and N-Cadherin expression levels in Flag-RGS2-transfected or Flag-tagged control vector-transfected (Flag-RGS2, -) HTR-8/SVneo cells. E Western blot analysis for E-Cadherin, Vimentin and N-Cadherin expression levels in RGS2 siRNA-transfected (siRGS2) or control siRNA-transfected (siRGS2, -) HTR-8/SVneo cells. F Wound healing assay in HTR-8/SVneo cells transfected with miR-3935 mimics or mimics negative control (mimics NC) and RGS2 siRNA (siRGS2) or control siRNA (siCon). G Statistical analysis for (F). H Transwell assay was performed to monitor cell invasion in HTR-8/SVneo cells transfected with miR-3935 mimics or mimics NC and siRGS2 or siCon. I Statistical analysis for (H). J Statistical analysis for migration of HTR-8/SVneo cells co-transfected with miR-3935 mimics or mimics NC and RGS2. K Statistical analysis for invasion of HTR-8/SVneo cells co-transfected with miR-3935 mimics or mimics NC and RGS2. L qRT-PCR analysis for E-Cadherin in HTR-8/SVneo cells co-transfected with miR-3935 mimics or mimics NC and RGS2. **p < 0.01 versus mimics negative control (NC) with siCon or mimics negative control (NC) without RGS2; ##p < 0.01 versus miR-3935 mimics with siCon; data represent the mean ± SD of three independent experiments; bar, 10 μm in (B), 80 μm in (F), 100 μm in (H)

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