Fig. 3

TRAF6 is a direct target of miR-3935. A The putative binding site of miR-3935 and RGS2 is shown. B Luciferase assay of HEK293T cells co-transfected with firefly luciferase constructs containing the TRAF6 wild-type 3’-UTRs and miR-3935 mimics, mimics NC, miR-3935 inhibitor or inhibitor NC. C Luciferase assay of HEK293T cells co-transfected with firefly luciferase constructs containing the TRAF6 mutated 3’-UTRs (mut) and miR-3935 mimics, mimics NC, miR-3935 inhibitor or inhibitor NC. D Western blot analysis for TRAF6 in miR-3935 mimics or mimics NC-transfected HTR-8/SVneo cells. E qRT-PCR analysis for RGS2 in HTR-8/SVneo cells co-transfected with TRAF6, CTR9 and miR-3935 mimics or mimics NC. **p < 0.01 versus mimics control (NC), inhibitor control (NC), or mimics control without TRAF6 or CTR9 (NC, TRAF6 -, CTR9 -); ##p < 0.01 versus mimics control with TRAF6 and without CTR9 (NC, TRAF6 + , CTR9 -); data represent the mean ± SD of three independent experiments