Fig. 2

Transcriptome analysis of miR-3935-regulated genes. A A heat-map of normalized expression levels of the altered genes measured by RNA-seq comparing negative miRNA control-transfected (NC) and miR-3935 mimics-transfected HTR-8/SVneo cells. Blue indicates low expression levels; red indicates high expression levels. B A volcano plot showing the altered genes in the presence of miR-3935 mimics. The number of genes is summarized in the table. C GO and pathway analysis of miR-3935-regulated genes measured by RNA-seq comparing negative miRNA control-transfected and miR-3935 mimics-transfected HTR-8/SVneo cells. D A table showing the three representative candidate genes, whose expression levels were significantly altered by miR-3935 mimics. E qRT-PCR analysis for RGS2, TRAF6 and CTR9 levels in miR-3935 mimics-transfected or negative miRNA control-transfected (NC) HTR-8/SVneo cells. F qRT-PCR analysis for RGS2, TRAF6 and CTR9 levels in miR-3935 inhibitors-transfected or negative miRNA control-transfected (NC) HTR-8/SVneo cells. **p < 0.01 versus mimics negative control (NC) or inhibitor negative control (NC); data represent the mean ± SD of three independent experiments