Fig. 2

OTOGL is nonessential for spermatogenesis in mice. A Schematic illustration of the targeting strategy for generating OTOGL deficiency in mouse testes. B The sequence of WT and Otogl KO alleles are shown, respectively. C Representative PCR genotyping results show that KO bands (669 bp) were detected larger than WT bands (472 bp). D Representative Western blot analysis of OTOGL protein levels in WT and KO adult testes. GAPDH served as a loading control. E RT-qPCR assays showing elevated Otogl mRNA levels in KO adult testes. Data are presented as mean ± SD, n = 3. *P < 0.01 by student’s t-test. F Average litter size of pups produced by Otogl KO and WT males mated with WT females. Data are presented as mean ± SD, n = 3. G Similar gross morphology of WT and Otogl KO testes. One unit on the ruler is 1mm. H Testis/body weight ratio of WT and Otogl KO adult testes. Data are presented as mean ± SD, n = 3