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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Effects of low intensity pulsed ultrasound on expression of B-cell lymphoma-2 and BCL2-Associated X in premature ovarian failure mice induced by 4-vinylcyclohexene diepoxide

Fig. 3

Effect of 4-vinylcyclohexene diepoxide (VCD) on estrus cycles in mice. Vaginal smears were obtained and the estrus cycles were evaluated by 0.23% alkaline methylene blue staining. Representative photographs: a proestrus, b estrus, c metestrus, d diestrus (100 ×). e Three patterns of estrous cycles were graded with the severity of abnormality (I–III) as follows: I, normal II, irregular cycles with a prolonged diestrus and normal or prolonged estrus III, no cyclicity. Bars represent the average length (day) of proestrus (P), estrus (E), metestrus (M) and diestrus (D) per estrous cycle. f Total numbers of mice from each group categorized into the various estrous patterns (I–III)

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