Fig. 2
From: Overexpressed MPS-1 contributes to endometrioma development through the NF-κB signaling pathway
MPS-1 regulates proliferation, migration, invasion, apoptosis and cell cycle of EcESCs. A Transfection efficiency of MPS-1 siRNA was detected by qRT-PCR. B A representative western blotting image of MPS-1 after EcESCs were transfected with MPS-1 siRNA for 72 h (n = 2). C Based on the results of western blotting, knockdown efficiency of MPS-1 siRNA was analyzed by Image J software. D The effects of MPS-1 siRNA on proliferation were evaluated by CCK-8 assay. E, G The representative images of EcESCs apoptosis and cell cycle were depicted by flow cytometry after MPS-1 siRNA transfection for 72 h. F, H The apoptosis analysis and cell cycle analysis were based on the results of flow cytometry. I, K Transwell assays were used to determine the effects of MPS-1 siRNA on migration and invasion. The scale bar is 50 μm. J, L Cell count analysis of Transwell migration and invasion. NC, si-MPS-1 EcESCs transfected with negative control siRNA or MPS-1 siRNA (n = 4). Data are presented as mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)