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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Circular RNA hsa_circ_0081343 promotes trophoblast cell migration and invasion and inhibits trophoblast apoptosis by regulating miR-210-5p/DLX3 axis

Fig. 3

Hsa_circ_0081343 physically interacts with miR-210-5p and miR-210-5p directly targets DLX3 transcript. A: Expression levels of miR-545-3p, miR-210-5p, and miR-597-3p in FGR and healthy placental tissues (n = 37). B: The binding site of miR-210-5p on hsa_circ_0081343 sequence, and the mutant scheme of luciferase reporter vector containing linear hsa_circ_0081343 with mutated miR-210-5p binding site. C: miR-210-5p mimic co-transfection decreases the relative luciferase activity of luciferase reporter vector containing wild type linear hsa_circ_0081343 (wild type circ_0081343). D: miR-210-5p mimic transfection increases hsa_circ_0081343 level in RNAs enriched by AGO2. E: The binding site of miR-210-5p on 3′ UTR of DLX3, and the mutant scheme of luciferase reporter vector containing 3′ UTR of DLX3 with mutated miR-210-5p binding site. F: miR-210-5p mimic co-transfection decreases the relative luciferase activity of luciferase reporter vector containing wild type 3′ UTR of DLX3 (wild type 3′ UTR). G-H: Effect of the overexpression or knockdown of miR-210-5p or hsa_circ_0081343 on DLX3 protein level. *p < 0.05, when compared to miR-NC. The experiments in panels A, C, D, F, G, and H were independently performed three times

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