Table 1 Primer sequences for RT-qPCR
Gene | Forward primer (5′-3′) Reversed primer (5′-3′) | Melting temperature (Tm) |
|---|---|---|
GAPDH | TCATCAACGGCACAGTCAAGG TTCTGCATGGTGGTGAAGACG | 60.88 60.88 |
LDH-C | TTTAGCCTTTTCCTCAGCACTCC AGTCTAGGTTACAGCCACTTCC | 60.81 59.17 |
FGF-21 | TTCGGGACTGTGGGTCTGTCTC TTTGCAGGTGGGCTTCGGTG | 62.6 62.1 |