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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Evaluation of testicular glycogen storage, FGF21 and LDH expression and physiological parameters of sperm in hyperglycemic rats treated with hydroalcoholic extract of Securigera Securidaca seeds, and Glibenclamide

Fig. 2

Photomicrographs of spermatozoa stained with specific methods for different purposes. A) Aniline blue for differentiation of immature (a, with a blue stained head) and mature (b, unstained) sperms. B) Eosin-Y-0.05% that distinguishes live sperms with uncolored heads (a) from dead ones with red-stained heads (b). C) Acridine-Orange, which differentiates sperms with intact chromatin structure (a, with a green fluorescence illuminating head) from those with denatured chromatin (b with a reddish-orange head. D) Eosin-Nigrosine for detection of sperms with morphological abnormalities

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