Fig. 2From: Evaluation of testicular glycogen storage, FGF21 and LDH expression and physiological parameters of sperm in hyperglycemic rats treated with hydroalcoholic extract of Securigera Securidaca seeds, and GlibenclamidePhotomicrographs of spermatozoa stained with specific methods for different purposes. A) Aniline blue for differentiation of immature (a, with a blue stained head) and mature (b, unstained) sperms. B) Eosin-Y-0.05% that distinguishes live sperms with uncolored heads (a) from dead ones with red-stained heads (b). C) Acridine-Orange, which differentiates sperms with intact chromatin structure (a, with a green fluorescence illuminating head) from those with denatured chromatin (b with a reddish-orange head. D) Eosin-Nigrosine for detection of sperms with morphological abnormalitiesBack to article page