1Pools of at least 30 oocytes were collected after 0, 21, or 42 hours of IVM (HS/TN).
2Oocytes experienced either TN throughout the entire IVM or HS during the first half of IVM (HS/TN).
3The PDC localization plugin in the Image J processing program was used to calculate Pearson’s and Spearman’s correlation coefficient representing colocalization of BCL2L1 with BAX or BECN1 in indivual oocytes. As per French et al. , a Pearson’s R of 0. 72 and Spearman’s R of 0.63 is considered a high degree of colocalization.