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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Characterization of the effects of heat stress on autophagy induction in the pig oocyte

Fig. 3

Colocalization of BAX and BCL2L1. Oocytes that underwent either TN/TN IVM or HS/TN IVM were fixed and used for IHC to determine colocalization. Neither the time point during IVM nor the temperature treatment affected colocalization of antibody-labeled BCL2L1 and BAX in the oocyte. Based on level of fluorescence, there appeared to be a qualitative increase in BAX (red) at 21 h of IVM under HS compared to 21 h of IVM at TN conditions, though there appeared to be no effect on the abundance of BCL2L1 (green; A). The comparison between 21-h TN (B), 21-h HS (C), or negative control (D) shows that there was punctate yellow signal representing colocalization in both TN and HS oocytes. For A, the white bar in images in the left and middle columns represent 50 μm, and the white bar in images in the right column represents 20 μm. The white arrows point to areas of colocalization. The images in the right column are magnified 5× images from the same oocyte from the middle column

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