Fig. 2
From: Characterization of the effects of heat stress on autophagy induction in the pig oocyte
Heat stress exacerbates the decrease of LC3B-II in the oocyte. Oocytes collected from aspirated pig follicular fluid underwent in vitro maturation (IVM) in either TN conditions throughout the entire 42 h maturation period or HS conditions during the first half of IVM (HS/TN). Representative western blot for LC3B from oocytes having undergone TN or HS conditions in IVM after 21 or 42 h or collected before IVM (A). There was decreased abundance of cleaved LC3B-II at 21 h of HS IVM compared with 21 h of TN IVM (B). Asterisks denotes significant difference (P < 0.05) from control