Fig. 1
From: Characterization of the effects of heat stress on autophagy induction in the pig oocyte
Heat stress during the first half of IVM increases the abundance of ATG12 in complex with ATG5. Oocytes collected from aspirated pig follicular fluid underwent in vitro maturation (IVM) in either TN conditions throughout the entire 42-h maturation period (TN/TN), HS conditions during the second half of IVM (TN/HS), or HS conditions during the first half of IVM (HS/TN). Representative western blots for BECN1, ATG12-ATG5 complex, BCL2L1, LC3B, and α-tubulin (A) from oocytes collected before IVM (GV) and after 42 h of IVM. There was increased abundance of ATG12 in complex with ATG5 after 42 h of IVM under the HS/TN temperature treatment compared to the TN/TN and TN/HS (B). Different superscripts within the same protein denotes significant difference (P < 0.05)