Tumor susceptibility gene 101 is required for the maintenance of uterine epithelial cells during embryo implantation

Table 1 Primers used for genotyping and RT-PCR analyses

Gene	Sequence (5′-3′)	Annealing temperature (°C)	No. of cycles	Product size (bp)
Tsg101 wildtype	F: CCG TGA TCT CTT GAT TCT TCT CC R: CCT GCT CTT TAC TGA AGG CTC	58	35	482
Tsg101 floxed	F: CCG TGA TCT CTT GAT TCT TCT CC R: GAA ATC CAC CTG CCT CTG CCT C	58	35	482
Ltf^iCre transgene	F: AAC TAG CAC ACC TGG TTG AGG R: CAG GTT TTG GTG CAC AGT CA	60.5	10	215
Des	F: CAA AGG GGT TCT GAA GTC CA R: GAA AAG TGG CTG GGT GTG AT	59	28	198
Krt12	F: GTC TCA TCC CAG GTT CAG GA R: TGC AAT GAA GAC CAG CAG AG	59	26	231
Rpl7	F: TCA ATG GAG TAA GCC CAA AG R: CAA GAG ACC GAG CAA TCA AG	59	28	246
Tsg101	F: ATG GCG GTG TCC GAG AGT CAG R: TTG ACA GTT TGA CGG ACG GT	55	33	80
Ripk1	F: GAA GAC AGA CCT AGA CAG CGG R: CCA GTA GCT TCA CCA CTC GAC	58	35	182
Ripk3	F: CAC ATA CTT TAC CCT TCA GA R: TCA GAA CAG TTG TTG AAG AC	58	35	172
Mlkl	F: GAC CAA ACT GAA GAC AAG TA R: CTC ACT ATT CCA ACA CTT TC	57	35	114
Aqp8	F: GGG GCA GCC TTT GCC ATC GT R: AAG AGG CCA GCC AGG AGG GG	59	28	296

ISSN: 1477-7827