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Table 1 Primers used for genotyping and RT-PCR analyses

From: Tumor susceptibility gene 101 is required for the maintenance of uterine epithelial cells during embryo implantation

Gene Sequence (5′-3′) Annealing temperature
(°C)
No. of cycles Product size (bp)
Tsg101
wildtype
F: CCG TGA TCT CTT GAT TCT TCT CC
R: CCT GCT CTT TAC TGA AGG CTC
58 35 482
Tsg101 floxed F: CCG TGA TCT CTT GAT TCT TCT CC
R: GAA ATC CAC CTG CCT CTG CCT C
58 35 482
LtfiCre
transgene
F: AAC TAG CAC ACC TGG TTG AGG
R: CAG GTT TTG GTG CAC AGT CA
60.5 10 215
Des F: CAA AGG GGT TCT GAA GTC CA
R: GAA AAG TGG CTG GGT GTG AT
59 28 198
Krt12 F: GTC TCA TCC CAG GTT CAG GA
R: TGC AAT GAA GAC CAG CAG AG
59 26 231
Rpl7 F: TCA ATG GAG TAA GCC CAA AG
R: CAA GAG ACC GAG CAA TCA AG
59 28 246
Tsg101 F: ATG GCG GTG TCC GAG AGT CAG
R: TTG ACA GTT TGA CGG ACG GT
55 33 80
Ripk1 F: GAA GAC AGA CCT AGA CAG CGG
R: CCA GTA GCT TCA CCA CTC GAC
58 35 182
Ripk3 F: CAC ATA CTT TAC CCT TCA GA
R: TCA GAA CAG TTG TTG AAG AC
58 35 172
Mlkl F: GAC CAA ACT GAA GAC AAG TA
R: CTC ACT ATT CCA ACA CTT TC
57 35 114
Aqp8 F: GGG GCA GCC TTT GCC ATC GT
R: AAG AGG CCA GCC AGG AGG GG
59 28 296