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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice

Fig. 2

OTOGL is nonessential for spermatogenesis in mice. (A) Schematic illustration of the targeting strategy for generating OTOGL deficiency in mouse testes. (B) The sequence of WT and Otogl KO alleles are shown, respectively. (C) Representative PCR genotyping results show that KO bands (669 bp) were detected larger than WT bands (472 bp). (D) Representative Western blot analysis of OTOGL protein levels in WT and KO adult testes. GAPDH served as a loading control. (E) RT-qPCR assays showing elevated Otogl mRNA levels in KO adult testes. Data are presented as mean ± SD, n = 3. *P < 0.01 by student’s t-test. (F) Average litter size of pups produced by Otogl KO and WT males mated with WT females. Data are presented as mean ± SD, n = 3. (G) Similar gross morphology of WT and Otogl KO testes. One unit on the ruler is 1mm. (H) Testis/body weight ratio of WT and Otogl KO adult testes. Data are presented as mean ± SD, n = 3

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