Fig. 4

Involvement of sPIF in the endometrial control of trophoblast invasion by ESCs. PIF improves the endometrial control of trophoblastic migration. A Transwell migration assays of HTR-8/SVneo cells were performed as described in the Materials and Methods. B Representative microphotographs of 12 separate experiments showing fixed and DAPI stained HTR-8/SVneo cells on the bottom side of the transwell membrane. HTR-8/SVneo cells were cultivated with conditioned medium (CM) from decidualized ESCs exposed for 15 days in control condition (a) or with 50 nM sPIF (b). C HTR-8/SVneo cells were suspended in the presence of CM from decidualized ESCs having been exposed for 15 days in presence or absence of sPIF (50 nM) or in the presence of control medium, supplemented or not with FCS 10% or adiponectin (250 ng/ml). The data are presented as the mean ± SEM of 3–12 separate experiments. ***: P < 0.001 (Wilcoxon test) for sPIF vs. the control (lacking sPIF)