Fig. 3

Effect of sPIF on human ESC motility. Human ESCs were cultured in DMEM/F12 medium supplemented with E2, P4 and (in some experiments) 50 nM sPIF for 15 days. Wound width was analyzed 0 and 24 h after wounding, as described in the Materials and Methods. The wound width data are expressed as the mean ± SEM of 5 separate experiments. *: P < 0.05 (Wilcoxon test) for sPIF vs. the control (lacking sPIF)