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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Preimplantation factor modulates trophoblastic invasion throughout the decidualization of human endometrial stromal cells

Fig. 1

Effect of sPIF on human ESC decidualization. Human ESCs were cultured in DMEM/F12 medium supplemented with E2, P4 and (in some experiments) 50 nM sPIF for 15 days. A-C Total RNA was extracted after three (D3), eight (D8), and 15 days (D15) of cell decidualization. mRNA expression levels of prolactin (A), IGFBP-1 (B), and connexin-43 (C) were quantified by RT-qPCR, as described in the Materials and Methods section. The data are presented as the mean ± SEM of 6 to 10 separate experiments. D Prolactin secretion into the endometrial supernatants was measured after 3 days (D3), 8 days (D8), and 15 days (D15) of cell differentiation, as described in the Materials and Methods. The data are presented as the mean ± SEM of 8 to 13 separate experiments. The control values on D3, D5, D8, D11, D13, and D15 were 46.5 ± 8.7, 130.7 ± 22.5, 346 ± 110, 419 ± 105, 494.5 ± 77 and 676.4 ± 102 mIU/g protein, respectively. *: P < 0.05; **: P < 0.01; ***: P < 0.001 (Wilcoxon test); (a) Prolactin secretion in presence of sPIF vs. the control (lacking sPIF); (b) Prolactin secretion at D15 vs. D3 for control experimental condition (lacking sPIF)

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