Fig. 13

AMHRII levels and localization in blastocysts. A Fixed blastocysts were stained with AMHRII antibody bound by Alexa Fluor-488 conjugated secondary antibody (green). Hoechst stain was used as the nuclear counterstain (blue). Column a) is the nuclear stain, column b) reflects AMHRII localization and amount, and column c) is an overlay of both channels. The last two rows represent the positive control (bovine COCs) and the negative control (blastocysts cultured in Alexa Fluor-488 without primary antibody). B Relative quantification of AMHRII protein levels in blastocysts (n = 10) determined by immunofluorescence. Bars represent the mean intensity of AMHRII in each blastocyst determined by intensity of Alexa Fluro 488-conjugated antibody measured by ImageJ software. * represents statistical significance p < 0.05. Error bars represent +/− SEM (n = 10 blastocysts)