Fig. 12

AMH immunofluorescence localization in blastocysts. A Blastocysts were fixed and stained with AMH antibody bound by Alexa Fluor-488 conjugated secondary antibody (green). Hoechst stain was used as the nuclei counterstain (blue). Column a) is the nuclear stain, column b) reflects the AMH expression, and column c) is an overlay of both the blue and green channels. The last two rows represent the positive control (bovine COC) and the negative control (blastocysts cultured in Alexa Fluor-488 without primary antibody). B Relative quantification of AMH protein levels in blastocysts (n = 10) determined by immunofluorescence measure. Bars represent the mean intensity of AMH in each blastocyst determined by intensity of Alexa Fluro 488-conjugated antibody measured with the ImageJ software. * represents statistical significance p < 0.01. Error bars indicate +/− SEM (n = 10 blastocysts)