Fig. 5
From: MicroRNA-30a-5p promotes differentiation in neonatal mouse spermatogonial stem cells (SSCs)
Immunocytochemistry for identifying C-Kit differentiation marker 48 h after transfection. a: SSCs were immunostained with antibody for C-Kit marker (green). DAPI was used for nuclear staining (blue). The images showed that the expression of C-Kit protein increased significantly in the inhibitor group compared to other groups. (Scale bars = 100 μm). b: Graph is indicative of the quantified area with fluorescent staining for C-Kit marker. Experiments were performed in triplicate. There were 2 × 105 cell/cm2 of SSCs in each group.The results are reported as mean ± SD ***p < 0.001