Fig. 4
From: MicroRNA-30a-5p promotes differentiation in neonatal mouse spermatogonial stem cells (SSCs)
Immunocytochemistry for identifying STRA8 differentiation marker 48 h after transfection. a: SSCs were immunostained with antibody for STRA8 marker (green). DAPI was used for nuclear staining (blue). The images showed that the expression of STRA8 protein increased significantly in the inhibitor group compared to other groups. (Scale bars = 100 μm). b: Graph is indicative of the quantified area with fluorescent staining for STRA8 marker. Experiments were performed in triplicate. There were 2 × 105 cell/cm2 of SSCs in each group. The results are reported as mean ± SD ***p < 0.001