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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis

Fig. 4

Knockdown of Talin1 repressed ADS endometrial stromal cell proliferative and pro-angiogenetic capacity. a. Cell Counting Kit-8 (CCK-8) assay was performed to assess the effect of Sh-Talin1 on the adenomyotic eutopic and ectopic endometrial stromal cell proliferation. The histogram illustrated the cell viability of ADS_Eu_ESC and ADS_Ec_ESC determined by the CCK-8 assay at 96 h post-transfection. b. Effect of Talin1 knockdown on the colony formation ability of ADS_Eu_ESC and ADS_Ec_ESC. c. Evaluation on the pro-angiogenetic ability of ADS_Eu_ESC and ADS_Ec_ESC cells transfected with Sh-Talin1 through the capillary tube and network formation assay. Images were captured when HUVECs were cultured with the conditioned medium on Matrigel for 6 h. Branch points of capillaries were counted and analyzed. Scale bar = 100 μm. At least triplicate independent experiments were performed. All data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. HUVEC, human umbilical vein endothelial cells

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