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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis

Fig. 3

Talin1 was overexpressed in human ADS uterine endometrial tissue and cells. a. The relative expression of Talin1 mRNA was elevated in ADS_Euc (n = 28) and ADS_Ec (n = 28) endometrium, as detected by qRT-PCR and normalized against Ctrl_En (n = 22) endometrium. b. Western blot analysis for Talin1 protein relative expression levels in ADS eutopic and ectopic endometrium as well as the normal uterine endometrium. c. qRT-PCR analysis of Talin1 mRNA in ADS and normal uterine endometrial stromal cells. d. Western blot detection and gray value analysis of Talin1 protein in ADS and normal uterine endometrial stromal cells. β-actin was used as a loading control. e-f. Evaluation on the upregulated efficiency of Talin1 in ADS endometrial stromal cells. g-h. Evaluation on the knockdown of Talin1 in ADS endometrial stromal cells. Data were presented as mean ± SD. NS no significance, *P < 0.05, **P < 0.01, ***P < 0.001. Ctrl_En, normal uterine endometrium as control; ADS_Euc, eutopic endometrium of adenomyosis; ADS_Ec, ectopic endometrium of adenomyosis; Ctrl_ESC, normal uterine endometrial stromal cells as control; ADS_Eu_ESC, adenomyotic eutopic endometrial stromal cells; ADS_Ec_ESC, adenomyotic ectopic endometrial stromal cells. CNC, complete negative control (cells without any treatment); Ov-NC and Sh-NC, overexpression and knockdown vector for negative control, respectively; Ov-Talin1 and Sh-Talin1, overexpression and knockdown of Talin1, respectively

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