Fig. 1
β-E2 induced adenomyotic endometrial stromal cell proliferation in vitro. a. Cell proliferation rates of adenomyotic and normal endometrial stromal cells with or without β-E2. b. Numbers of formative colonies of adenomyotic and normal endometrial stromal cells with or without β-E2. c. and d. CCK-8 assay and colony formation assay were performed to evaluate the effects of β-E2 on the proliferation of ADS eutopic and ectopic endometrial stromal cells. e. and f. Western blot and gray value analysis of molecular markers for cell division and proliferation including PCNA and Ki67 in ADS endometrial stromal cells treated with E2 (10nM) or Fulvestrant (a selective estrogen receptor antagonist, 10 nM) or both or neither of them. GAPDH was used as a loading control. Data were presented as mean ± SD. NS no significance, *P < 0.05, **P < 0.01. ADS_Eu_ESC, adenomyotic eutopic endometrial stromal cells; ADS_Ec_ESC, adenomyotic ectopic endometrial stromal cells