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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Upregulated microRNA let-7a accelerates apoptosis and inhibits proliferation in uterine junctional zone smooth muscle cells in adenomyosis under conditions of a normal activated hippo-YAP1 axis

Fig. 1

The expression levels of let-7a, YAP1 and TAZ in JZ SMCs in the ADS group and control group. a The mRNA expression level of let-7a determined by RT-qPCR and normalized against that of U6. b The mRNA expression level of YAP1 determined by RT-qPCR and normalized against that of β-actin. c The mRNA expression level of TAZ determined by RT-qPCR and normalized against that of β-actin. d The protein expression levels of YAP1 and TAZ determined by western blotting and normalized against that of α-tubulin. e The relative fold protein expression of YAP1 normalized against α-tubulin. f The relative fold protein expression of TAZ normalized against α-tubulin. These experiments were performed two times with three replicates in each experiment. Significance was determined by Student’s t test; **p < 0.01, *p < 0.05

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