Fig. 5

CLU is upregulated in the placenta from PE deliveries. a, Fixed placental tissue sections were used for immunohistochemical analysis. Student’s t-test, n = 6. b, LPS stimulation leads to increased expression of CLU in TEV-1 cells. The upper part shows a representative western blot band and below is the relative quantification after normalization. One-way ANOVA, n = 3. c, Total placental RNA was extracted for relative quantification of CLU mRNA levels by qRT-PCR (n = 19). d, Total placental RNA was extracted for relative quantification of MMP9 mRNA levels (n = 19). e, Placental CLU gene expression was negatively correlated with MMP9 via Pearson’s correlation. f, CLU inhibits TEV-1 cell invasion. The left panel is a representative micrograph of the Matrigel Transwell assay treated with CLU. The right panel represents quantitative results of cell invasion, and data are presented as the mean ± SD and were analysed by one-way ANOVA, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001. PE = Preeclampsia, N=Normal pregnancy, CLU=Clusterin, MMP9 = Matrix metalloproteinases 9