Fig. 4

Effect of WAPL depletion on the spindle/chromosome structure in porcine oocytes. a Representative images of spindle morphologies and chromosome alignment in control and WAPL-KD oocytes. Scale bar, 5 μm. b The proportion of defective spindles was recorded in control (n = 73) and WAPL-KD (n = 82) oocytes. c The proportion of misaligned chromosomes was recorded in control (n = 73) and WAPL-KD (n = 82) oocytes. Data of (b) and (c) were presented as mean percentage (mean ± SEM) of at least three independent experiments. ** P < 0.01, *** P < 0.001