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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: WAPL orchestrates porcine oocyte meiotic progression via control of spindle assembly checkpoint activity

Fig. 3

Effect of WAPL depletion on the protein level and localization of BUB3 in porcine oocytes. a Immunoblotting analysis of BUB3 in control and WAPL-KD oocytes. 120 oocytes were collected and immunoblotted for BUB3, WAPL and α-tubulin. The band intensity of WAPL and BUB3 was normalized with α-tubulin. b Representative images of BUB3 localization in control or WAPL-KD oocytes. Porcine oocytes at Pro-M I stage were immunostained for BUB3 and WAPL, and counterstained for DNA. Scale bar, 2.5 μm

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