Fig. 2

Effect of WAPL depletion on the meiotic progression of porcine oocytes. a Representative images of chromosomes in oocytes after GVBD in control and WAPL-KD groups. Scale bar, 2.5 μm. b The incidence of GVBD was quantified in control (n = 82) and WAPL-KD (n = 85) oocytes. c Representative images of oocytes with first polar body at time point of 40 h following in vitro culture were shown in control and WAPL-KD groups. Scale bars, 100 μm, 25 μm. d Quantitative analysis of polar body extrusion rate was shown in control (n = 107) and WAPL-KD (n = 107) oocytes at consecutive time points from 38 to 44 h during in vitro maturation