Fig. 1

Subcellular localization and protein expression of WAPL during porcine meiotic maturation. a Representative localization of endogenous WAPL in porcine oocytes. Porcine oocytes at different developmental stages were immunostained with WAPL antibody and counterstained with Hoechst. Scale bar, 2.5 μm. b Representative image of co-localization of WAPL with kinetochores in porcine oocytes. Porcine oocytes at pro-metaphase I (Pro-M I) stage were immunostained with WAPL and CREST antibodies and counterstained with Hoechst. Scale bar, 2 μm. c Protein levels of WAPL during porcine oocyte meiotic maturation corresponding to GV, GVBD, metaphase I (M I) and metaphase II (M II) stages were examined by immunoblotting. The blots were probed with WAPL and GAPDH antibodies, respectively