Fig. 8

The role of NAC in FLC-induced ROS levels, AKT activation and autophagy levels. Sertoli cells were pretreated with 5 mM NAC for 2 h and then exposed to FLC. a The effect of FLC-induced ROS level was observed. b Western blot detected AKT signaling pathway proteins (AKT, p-AKT) and autophagy-activating proteins (LC3, Beclin-1, P62). Ns represents no significance, *P < 0.05 significantly different from the corresponding group