Fig. 2

Misaligned chromosomes in MI- and MII-stage oocytes from serum-ACA-positive mice. In the HA + CFA group, most MI- (a) and MII-stage (b) oocytes showed severely misaligned chromosomes, while in the control groups, most oocytes showed normal chromosome alignment. None of the oocytes from the three groups exhibited IgG fluorescence. DNA (blue), tubulin (green), IgG (red). Scale bars: 10 μm. c Metaphase plate width was determined by measuring the axis distance between the two lines at the edges of the DNA. Data are expressed as mean ± SD of ten MI-stage oocytes in each group. **P < 0.01; ***P < 0.001. d Positive and negative control for IgG stain. Mouse ovarian tissue sections from HA + CFA and Saline groups were stained with the anti-mouse IgG. The red fluorescence of IgG, predominantly distributed in the follicular fluid, was observed in mice from the HA/CFA group, and no fluorescence of IgG was visualized in mice from the Saline group, which were served as positive and negative controls for IgG stain, respectively (with an original magnification × 200)