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Table 1 Schematic for the study design

From: Effect of resveratrol on mouse ovarian vitrification and transplantation

 

Methods

Day (after transplantation)

Saline (number of mice)

5 mg/kg (number of mice)

15 mg/kg (number of mice)

45 mg/kg (number of mice)

Fresh

6

     

vitrification

6

     

ExperimentI

 

3 d

6

6

6

6

HE/E2/FSH

7 d

6

6

6

6

 

21 d

6

6

6

6

ExperimentII

TUNEL/IHC

3 d

6

  

6

7 d

6

  

6

Western blot

3d

6

  

6

7d

6

  

6

RT-PCR

3d

8

  

8

7d

8

  

8

IVM/IVF

21d

6

  

6

  1. Experiment I Evaluation of the best concentration of resveratrol, Experiment II Further evaluation of the effects of resveratrol and exploration of the mechanisms